Birmingham MP Richard Burden, Labour's official transport spokesman, hit out at Deirdre Alden's claims that Birmingham's £23 million plan to encourage cycling was discriminatory.
Coun Alden (Con Edgbaston) claimed in September cycling was "a discriminatory form of transport" with women who wish to wear modest clothing unlikely to partake.
The comments sparked a major backlash, including from charity Sustrans, but some of her claims have been backed up by city council assessments suggesting many feel excluded.
Meanwhile, ministers have called for investment in cycling to be increased to at least £10 per head – and claim it would save the NHS billions of pounds a year.
Mr Burden (Lab Northfield), a Shadow Transport Minister, said: "In my own town, Birmingham, a prominent Conservative councillor is on the record saying that cycling is discriminatory against women, particularly women from ethnic minorities. Fortunately, most people in Birmingham do not share that view."
He told MPs: "There are huge benefits to cycling. In particular, it improves people's health – physical inactivity costs the NHS between £1 billion and £1.8 billion every year – and protects the environment by tackling air pollution and congestion in our towns and cities."
Birmingham City Council's own "impact equality assessment", produced in 2013 as part of a bid for government funding to help pay for the city's cycle scheme, confirms that some ethnic groups are currently more likely to cycle than others and states the scheme is designed to encourage "excluded" groups to start cycling.
It states: "The proposals will make cycling a more attractive travel option for short trips, including a range of communities and social groups, many of which are ethnically mixed or socially deprived, and which may not have a cycling culture at the moment.
"Inclusion of women will be encouraged through initiatives such as Women on Wheels as part of the supporting measures package."
Coun Alden was criticising a £23 million scheme intended to transform cycling in Birmingham.
The Department for Transport gave Birmingham £17 million to become a "Cycle City", with another £6 million coming from the council, and the cash will allow the creation of a network of safe new routes aimed at tempting commuters out of their cars.
Money will be used to buy 5,000 bikes and set up cycle hubs – with 2,000 of them given away, and key commuter routes, including Hagley Road and Bristol Road, will have dedicated cycle lanes and safety improvements will be made at junctions.
In the same debate, a Black Country MP told the Commons that getting Britain cycling could save the NHS £17 billion.
Ian Austin (Lab Dudley North), co-chair of the All Party Parliamentary Cycling Group, said the benefits of increasing investment in cycling would include a reduction in heart disease, diabetes and colon cancer rates.
He told MPs the all-party group had produced a report following an inquiry which heard from hundreds of witnesses.
"A central recommendation is for long-term, dedicated funding of £10 per head per year, rather than limited funding for eight cities for a couple of years.
"We want ten per cent of journeys to be made by bike by 2025 – the figure was less than two per cent in 2011 – and we call for lower speed limits in urban areas. We want more effective enforcement of the law, we want children to be taught to ride at school, we want more segregated cycle lanes, and we want cycling to be considered properly as part of the urban planning process."
And he highlighted a study which found that getting more people cycling would provide economic benefits.
"The possible benefits range from saving the NHS £17 billion to increasing the mobility of the nation's poorest families by 25 per cent.
"Traffic delays in London cost £1.5 billion a year. An increase in cycling would tackle congestion and pollution, and would make our roads safer and our transport system more efficient.
"It would enable people on low incomes to travel more easily, would make our town and city centres more pleasant places, and would support local economies."
Funding aimed specifically at Birmingham, Manchester, Leeds, Newcastle, Bristol, Cambridge, Oxford and Norwich was already taking funding above £10 per head in those cities, Transport Minister Robert Goodwill said.