This year's Royal Show, held at Stoneleigh Park in Warwickshire, will be the last, organisers have said.
The annual agricultural show has been hit in recent years by the foot-and-mouth disaster in 2001, the very wet summer and flooding in 2007 and the arrival of bluetongue into the UK.
The Royal Agricultural Society of England (Rase) said the event was no longer economically viable and would not continue in its present form after this year.
This year's Royal Show, the 160th, will still go ahead this summer. The chairman of the trustees, Hugh Oliver Bellasis, said the decision was a real challenge, but the society had been left with little choice.
"In the aftermath of the foot-and mouth-disaster in 2001, closely followed by very bad weather two years ago and bluetongue last year, the event has struggled - both financially and in its ability to attract both farming and non-farming visitors," he said.
But he urged people to come to the final show between July 7 and 10, enjoy the livestock, horses, technical features and shopping and "bid a fond farewell to an event that has long been held in great affection by farmers and others who work and live in the British countryside".
The Rase intends to create a new programme of events from 2010, including taking elements of the Royal Show for inclusion in other events.