Five men and six women are aiming for the top spot this year, after coming runners-up last year and third place in 2013.
Team co-ordinator James Cruse said: "We are really hoping to snatch victory this year.
"It's our third year entering and we are all really competitive so we're really putting the training in."
JW Hinks will be fundraising for Birmingham-based charity St Basil's which works with young people who are homeless or at risk of homelessness.
Ahead of this year's half marathon on October 18, race organisers are encouraging Business Challenge teams to make their own short motivational video with the winning team awarded a training session with a professional coach.
Business Challenge teams must be a minimum of four people and a maximum of eight but companies can enter as many teams as they want in male, female or mixed-sex categories.
Mixed teams must have a minimum of two men and two women.
Team members must be 16 years of age or over on race day and placings are based on the accumulated first four finishing times in each team.
Every team that enters 12 or more employees will receive their own personalised company engraved plaque.
Visit www.greatrun.org/Birmingham for more details and to enter the Great Birmingham Run.