A five-year-old girl who was rescued from a car that plunged into the River Avon at Evesham has died.
Gabrielle Grady and her brother Ryan, six, were rescued on Thursday after the vehicle, a silver Vauxhall Vectra estate, entered the water from a field at Boat Lane in Evesham.
Their father, Christopher Grady, was remanded in custody at Worcester Magistrates' Court on Saturday, charged with the attempted murder of his children.
Grady, 41, of no fixed abode, is due to appear at Worcester Crown Court on Thursday.
Police said Gabrielle's death meant the incident was now being treated as a murder investigation.
The child had been in a critical condition since she and her brother were rescued and airlifted to Birmingham Children's Hospital.
Ryan was also critically ill when first admitted to hospital but was said to be "recovering quite well."
West Midlands Ambulance Service said the driver and Ryan were already out of the submerged car when paramedics arrived, but Gabrielle was trapped for almost two hours before she was pulled free by police divers.
A West Mercia Police spokesman said: "Gabrielle passed away peacefully at around 1.30pm today [Sunday], surrounded by her family."
Detective Superintendent Steve Cullen, who is leading the investigation, said: "We are very sad to hear this terrible news and all our thoughts are with Gabby's family at this difficult time."