Nearly a fifth of Birmingham's secondary schools failed to reach Government targets in literacy, numeracy and science for 14-year-olds.
Fourteen of the city's 77 schools are listed among the 200 worst performing in the country for Key Stage Three tests, in league tables published today.
How did your local school perform? Full league tables published in today's Birmingham Post
St Alban's CofE in Highgate propped up the KS3 national table and was also the worst of nine schools - which included a Walsall secondary - failing to get pupils to the level of competence expected of a primary school leaver.
Birmingham as a whole missed the average point score per pupil needed to pass KS3 tests.
The LEA last night claimed St Alban's was doing well in tough circumstances and defended the city's record compared with other similar authorities. It also maintained the number of schools where 14-year-olds failed to reach Government floor targets in English and mathematics was falling.
Chris Clifford, regional director of business lobby group the CBI, said: "Unfortunately this confirms the fears we have.
"We are concerned our members are still telling us they need pupils coming out of school able to read, write and add up," he added.