Business rates will be cut for one year from October, meaning a tax reduction for over 500,000 small businesses in England.
Small businesses will be helped to expand by doubling the annual investment allowance to £100,000.
Entrepreneurs' relief for Capital Gains Tax will be doubled to £2 million on which the lower rate of 10% will be taxed.
The Chancellor said he would provide £100 million to pay for vital repairs to local roads throughout the country following the recent bad weather and £285 million to pay for improvements on motorways.
To boost a low-carbon economy, the Government will set up a new Green Investment Bank, controlling £2 billion of equity. Half the cost will come from asset sales, with the rest matched by private investment.
The fund will focus on green transport and energy, including offshore wind power, with £60 million offered to develop ports hosting manufacturers of offshore wind turbines.
The Chancellor will offer help to the computer games sector similar to the aid given the British film industry. The Government will set up a £35 million University Enterprise Capital Fund to support university innovation and spin-out companies.
A University Modernisation Fund will give a one-off funding boost of £270 million in 2010/11, creating 20,000 more university places starting this
The cost of the £2.5 billion overall one-off growth package will be met from existing budgets and by higher revenues from the bankers' bonus tax.
The Budget will bring in additional tax of half a billion pounds each year said Mr Darling.
Parents of one- and two-year-old children will be helped by increasing by £4 a week money paid through Child Tax Credit from 2012.
The pensioners' higher Winter Fuel Payment of £250, and £400 for the over-80s will be guaranteed for another year.
The country must choose whether to support those whose policies will suffocate our recovery or a Government which has been right about the recession and the recovery, declared the Chancellor.