Dudley Zoo's suspended chief executive Peter Suddock has left the post by mutual agreement after 22 years in the role.
The well-known Midland business leader, 68, has officially retired from his job after the zoo announced his suspension earlier this month.
Mr Suddock, who has been chief executive of the zoo since 1993, had been suspended on full pay pending an internal inquiry thought to revolve around finances.
His partner Jill Hitchman, who has worked as the zoo's head of media and communications, is also no longer in her role.
Mr Suddock was suspended while an internal investigation was carried out but a statement issued by the zoo today said: "By mutual agreement and after 22 years in post, Peter Suddock has retired from his position as chief executive officer of Dudley Zoological Gardens."
The statement added no further detail over the circumstances of his departure or whether a financial settlement had been reached.
The zoo previously said his suspension had not constituted disciplinary action and "whether or not disciplinary action is taken will depend upon the outcome of the investigation".
Mr Suddock's career began in the engineering industry before he became chief executive of registered charity Dudley Zoological Gardens in 1993.
He is also a former chairman of the Royal Wolverhampton NHS Trust and has been chairman of the Heart of England Attractions Group, a consortium of West Midlands leisure attractions, and forged links with zoo and leisure groups across the UK.
In May 2013, he was awarded the director of the year in the not-for-profit category in the West Midlands' Institute of Directors' annual awards.
Neither Mr Suddock nor Ms Hitchman could be contacted for comment and Zoo Trust chairman Neil Cutler also declined to comment.