Former Tory whip Michael Fabricant is to lead opposition to the planned high speed rail line when it is debated in the Commons later this month.
The MP, Vice-Chairman of the Conservative Party, will attempt to block the second reading of the High Speed Rail Bill when it comes to the Commons on April 28.
It follows the Government’s announcement that the second reading will be held on just one day, despite demands for at least two days of debate.
Mr Fabricant, MP for Lichfield in Staffordshire, has tabled an amendment which, if passed, would require the Government to draw up a new route allowing a high speed rail line to be built at lower cost and causing less damage to the environment.
The House of Commons Environmental Audit Committee has also warned that better safeguards need to be implemented if harmful environmental impacts of HS2 are to be minimised.
Mr Fabricant said: “I have taken the unusual step of tabling an amendment to the Second Reading which, if passed, would defeat the Bill.
“I hope it might attract Labour as well as Conservative votes if it is chosen to be voted on by the Speaker. In any event, it points out clearly in House of Commons papers the arguments against the Bill.
“I know that the majority of MPs of all parties support the principle of HS2 and that the Bill’s Second Reading is likely to be passed with a huge majority with Labour support, though many MPs are uneasy about the way HS2 is being implemented.
“I also know that Labour MPs as well as Conservatives and Liberal-Democrats are concerned about the cost, the environmental damage, and its initial focus on southern England and the midlands rather than the north.
“I have, therefore, phrased the amendment to support the principle of HS2 and tempt Labour votes, but it would still defeat the Bill for the reasons given if it is passed.”
Labour is to whip its MPs to support the HS2 Bill.
The Environmental Audit Committee urged Ministers to consider reducing the maximum 225mph speed of the HS2 trains, to cut the level of pollution caused.
Building HS2 could lead to some plant species becoming endangered while emission savings from the high-speed rail scheme are likely to be “relatively small at best”, according to the Committee.
Better safeguards needed to be implemented if harmful environmental impacts of HS2 were to be minimised, the report from the House of Commons Environmental Audit Committee said.
The MPs said that Parliament, in its capacity as the planning authority for the high-speed rail project, should ensure that everything possible was done to minimise damage to ancient woodlands and Sites of Special Scientific Interest.
Where loss was genuinely unavoidable, compensation should be applied to the fullest extent possible.
The committee also said that consideration should be given to Launching the report, the committee’s chairman Joan Walley said: “So far the consultation process on HS2 has not fully addressed the many environmental concerns we have.
“The Government needs to show real commitment to dealing with the impact that HS2 will have on our countryside and wildlife.
“Ancient woodlands and other hard-to-replace sites of natural value should not be subordinated to crude economic calculations of cost and benefit.”